Welcome

This guide will walk you through analysis of NULISAseq proteomic data using R.

What is NULISAseqR?

NULISAseqR is an R package designed specifically for working with NULISAseq proteomic data. It provides:

  • Data Import: Read XML files from NULISAseq multiplex proteomic panels
  • Quality Control: Automated QC reporting and metrics
  • Visualization: Heatmaps, PCA plots, volcano plots, boxplots, etc.
  • Statistical Analysis: Differential expression testing and longitudinal analysis
  • Predictive Modeling: Linear models using protein expression to predict an outcome

Installation

New to R? If you haven’t installed R and RStudio yet, see the Installing R and RStudio guide in the Additional Resources page.

Install NULISAseqR from GitHub:

# Install devtools if needed
install.packages("devtools")

# Install NULISAseqR
devtools::install_github('Alamar-Biosciences/NULISAseqR', ref = 'main')

# Install other packages
install.packages("tidyverse")
install.packages("table1")
install.packages("ggVennDiagram")
install.packages("pROC")


Load the packages:

library(NULISAseqR)
library(tidyverse)

Getting Help

© 2025 Alamar Biosciences Bioinformatics Team
Generated with NULISAseqR version 1.4.0
Last updated: December 04, 2025